Ascorbate Oxidase antibody (HRP)

Catalog No. ABIN5596757

Supplier Product No.: 200-4348-0100

This Rabbit Polyclonal antibody specifically detects Ascorbate Oxidase in ELISA, WB and IP. It exhibits reactivity toward Pumpkin.

Quick Overview for Ascorbate Oxidase antibody (HRP) (ABIN5596757)

Target: Ascorbate Oxidase (AO)

Reactivity: Pumpkin

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ascorbate Oxidase antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Ascorbate Oxidase Antibody (HRP)

Supplier Product No.: 200-4348-0100

Supplier: Rockland

Cross-Reactivity (Details): Cross reactivity against Ascorbate Oxidase from other sources is unknown.

Purification: Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].

Immunogen:

Immunogen: Ascorbate Oxidase [cucurbita]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Ascorbate Oxidase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:3,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Ascorbate Oxidase

Target: Ascorbate Oxidase (AO)

Alternative Name: Ascorbate Oxidase

Background:

Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3

Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.

Gene Name: AAO

UniProt: P24792