CD31 antibody (C-Term)

Catalog No. ABIN5596763

Supplier Product No.: 100-401-gn4

This Rabbit Polyclonal antibody specifically detects CD31 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for CD31 antibody (C-Term) (ABIN5596763)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CD31 Antibody

Binding Specificity: C-Term

Supplier Product No.: 100-401-gn4

Supplier: Rockland

Purpose: CD31 Antibody

Cross-Reactivity (Details): CD31 PECAM1 antibody is directed against rat CD31 protein.

Characteristics: Synonyms: Rabbit anti-CD31 antibody, rabbit anti-PECAM-1 antibody, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD-31 Antibody, PECAM1 Antibody, CD31/PECAM1 Antibody

Purification: The product is delipidated and defibrinated antiserum.

Sterility: Sterile filtered

Immunogen:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of rat CD31 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 4 μg/mL

Application Note: CD31 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in size corresponding to CD31 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Western Blot Dilution: 1:500

ELISA Dilution: 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: Pecam1

Background: Background: Anti-CD31 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). CD31 (PECAM-1) is a 130 kDa platelet endothelial cell adhesion molecule encoded by the PECAM1 gene found on chromosome 17 in humans. CD31 is expressed on platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 plays a key role in modulation of integrin-mediated cell adhesion, leukocyte transendothelial migration, angiogenesis, apoptosis and macrophage phagocytosis. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. Anti-CD31 is ideal for researchers interested in Stem Cell Research, Epigenetics, and Cell-cycle Regulation research.

Gene ID: 29583

NCBI Accession: NP_113779

UniProt: Q3SWT0

Pathways: Regulation of Actin Filament Polymerization