APOA1 antibody

Catalog No. ABIN5596807

Supplier Product No.: 600-101-109

This anti-APOA1 antibody is a Goat Polyclonal antibody detecting APOA1 in WB, IHC, ELISA, IP and FM. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for APOA1 antibody (ABIN5596807)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Product Details anti-APOA1 Antibody

Supplier Product No.: 600-101-109

Supplier: Rockland

Purpose: APOLIPOPROTEIN A-I Antibody

Cross-Reactivity (Details): Typically less than 1 % cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.

Characteristics: Synonyms: goat anti-Apolipoprotein A-I Antibody, goat anti-APOA1 antibody, goat anti-APO-A1 antibody, goat anti-APOA-1 antibody, APOA1/APOC3 fusion gene antibody, Apolipoprotein A I precursor antibody, Apolipoprotein AI antibody, Apolipoprotein of high density lipoprotein antibody, ProapoA-I, Proapolipoprotein A-I, ApoA-I

Purification: Goat Anti-Apolipoprotein A-I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Sterility: Sterile filtered

Immunogen:

Immunogen: apoLipoprotein Type A-I was isolated from human plasma by density gradient centrifugation followed by HPLC purification, followed by repeated immunizations in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-apoLipoprotein antibodies have been tested by Western blot and IHC and are suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation, immunohistochemistry and for western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:20,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.   This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-APOA1 antibody (ABIN5596807)

Jani, Orlicky, Karimpour-Fard, Epperson, Russell, Hunter, Martin: "Kidney proteome changes provide evidence for a dynamic metabolism and regional redistribution of plasma proteins during torpor-arousal cycles of hibernation." in: Physiological genomics, Vol. 44, Issue 14, pp. 717-27, (2012) (PubMed).

Otis, Sahoo, Drover, Yen, Carey: "Cholesterol and lipoprotein dynamics in a hibernating mammal." in: PLoS ONE, Vol. 6, Issue 12, pp. e29111, (2012) (PubMed).

Martin, Epperson, Rose, Kurtz, Ané, Carey: "Proteomic analysis of the winter-protected phenotype of hibernating ground squirrel intestine." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 295, Issue 1, pp. R316-28, (2008) (PubMed).

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: APOA1

Background: Background: Anti-Apolipoprotein A-I antibody recognizes the gene product of APOA1. Apolipoprotein promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease. Anti-Apolipoprotein A-I is useful for researchers interested in cardiovascular research.

Gene ID: 335, 4557321

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism