APOA1 antibody

Catalog No. ABIN5596808

Supplier Product No.: 600-101-196

This Goat Polyclonal antibody specifically detects APOA1 in WB, IHC, ELISA and IP. It exhibits reactivity toward Mouse and has been mentioned in 10+ publications.

Quick Overview for APOA1 antibody (ABIN5596808)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-APOA1 Antibody

Supplier Product No.: 600-101-196

Supplier: Rockland

Purpose: Apolipoprotein A-I Antibody

Cross-Reactivity (Details): Typically less than 1 % cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards.

Characteristics: Synonyms: goat anti-Apolipoprotein A-I Antibody, goat anti-APOA1 antibody, goat anti-APO-A1 antibody, goat anti-APOA-1 antibody, APOA1/APOC3 fusion gene antibody, Apolipoprotein A I precursor antibody, Apolipoprotein AI antibody, Apolipoprotein of high density lipoprotein antibody, ProapoA-I, Proapolipoprotein A-I

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.

Sterility: Sterile filtered

Immunogen:

Immunogen: apoLipoprotein Type A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:250 - 1:500

Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

References for anti-APOA1 antibody (ABIN5596808)

Han, Tang, Guevara, Wei, Wietecha, Shao, Subramanian, Omer, Wang, OBrien, Marcovina, Wight, Vaisar, de Beer, de Beer, Osborne, Elkon, Chait: "Serum amyloid A impairs the antiinflammatory properties of HDL." in: The Journal of clinical investigation, Vol. 126, Issue 1, pp. 266-81, (2016) (PubMed).

Tóth, Szegedi, Varga, Juhász, Horváth, Borbély, Csibrány, Alföldi, Lénárt, Penke, Sántha: "Overexpression of Hsp27 ameliorates symptoms of Alzheimer's disease in APP/PS1 mice." in: Cell stress & chaperones, Vol. 18, Issue 6, pp. 759-71, (2014) (PubMed).

Fitz, Cronican, Saleem, Fauq, Chapman, Lefterov, Koldamova: "Abca1 deficiency affects Alzheimer's disease-like phenotype in human ApoE4 but not in ApoE3-targeted replacement mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 38, pp. 13125-36, (2012) (PubMed).

Tadin-Strapps, Peterson, Cumiskey, Rosa, Mendoza, Castro-Perez, Puig, Zhang, Strapps, Yendluri, Andrews, Pickering, Rice, Luo, Chen, Tep, Ason, Somers, Sachs, Bartz, Tian, Chin, Hubbard, Wong, Mitnaul: "siRNA-induced liver ApoB knockdown lowers serum LDL-cholesterol in a mouse model with human-like serum lipids." in: Journal of lipid research, Vol. 52, Issue 6, pp. 1084-97, (2011) (PubMed).

Champy, Le Voci, Selloum, Peterson, Cumiskey, Blom: "Reduced body weight in male Tspan8-deficient mice." in: International journal of obesity (2005), Vol. 35, Issue 4, pp. 605-17, (2011) (PubMed).

Chen, Burton, Song, McNamara, Langella, Cianetti, Chang, Wang: "An apoA-I mimetic peptide increases LCAT activity in mice through increasing HDL concentration." in: International journal of biological sciences, Vol. 5, Issue 5, pp. 489-99, (2009) (PubMed).

Hu, Abe-Dohmae, Tsujita, Iwamoto, Ogikubo, Otsuka, Kumon, Yokoyama: "Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo." in: Journal of lipid research, Vol. 49, Issue 2, pp. 386-93, (2008) (PubMed).

van der Hoorn, de Haan, Berbée, Havekes, Jukema, Rensen, Princen: "Niacin increases HDL by reducing hepatic expression and plasma levels of cholesteryl ester transfer protein in APOE*3Leiden.CETP mice." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 11, pp. 2016-22, (2008) (PubMed).

van der Hoogt, de Haan, Westerterp, Hoekstra, Dallinga-Thie, Romijn, Princen, Jukema, Havekes, Rensen: "Fenofibrate increases HDL-cholesterol by reducing cholesteryl ester transfer protein expression." in: Journal of lipid research, Vol. 48, Issue 8, pp. 1763-71, (2007) (PubMed).

Allen, Piontek, Garrett-Mayer, Garcia-Gonzalez, Gorelick, Germino: "Loss of polycystin-1 or polycystin-2 results in dysregulated apolipoprotein expression in murine tissues via alterations in nuclear hormone receptors." in: Human molecular genetics, Vol. 15, Issue 1, pp. 11-21, (2005) (PubMed).

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: Apoa1

Background: Background: Anti Apolipoprotein A-I antibody recognizes the gene product of APOA1. Apolipoprotein promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease. This antibody is suitable for cardiovascular research.

Gene ID: 11806

NCBI Accession: NP_033822

UniProt: Q00623

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism