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Acid Phosphatase antibody (HRP)

Rockland 200-4340-0100 ACP Reactivity: Potato WB, ELISA, DB Host: Rabbit Polyclonal HRP
Rockland
Catalog No. ABIN5596813
Supplier Product No.: 200-4340-0100
  • Target See all Acid Phosphatase (ACP) Antibodies
    Acid Phosphatase (ACP)
    Reactivity
    • 15
    • 11
    • 5
    Potato
    Host
    • 31
    Rabbit
    Clonality
    • 31
    Polyclonal
    Conjugate
    • 10
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Acid Phosphatase antibody is conjugated to HRP
    Application
    • 31
    • 13
    • 13
    • 13
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA, Dot Blot (DB)
    Supplier Product No.
    200-4340-0100
    Supplier
    Rockland
    Purpose
    Acid Phosphatase Antibody Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].
    Characteristics
    Synonyms: rabbit anti-Acid Phosphatase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody
    Purification
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Acid Phosphatase [Potato]

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product ACP Primary Antibody
  • Application Notes

    Application Note: Anti-Acid Phosphatase Peroxidase Conjugated Antibody has been tested by dot blot and western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:4,000 - 1:20,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer
    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    Acid Phosphatase (ACP)
    Alternative Name
    Acid Phosphatase (ACP Products)
    Background
    Background: The catalytic activity of Acid Phosphatase is phosphate monoester + H2O = an alcohol + phosphate.
    UniProt
    Q6J5M8
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