Acid Phosphatase antibody (HRP)

Catalog No. ABIN5596813

Supplier Product No.: 200-4340-0100

This anti-Acid Phosphatase antibody is a Rabbit Polyclonal antibody detecting Acid Phosphatase in WB, ELISA and DB. Suitable for Potato.

Quick Overview for Acid Phosphatase antibody (HRP) (ABIN5596813)

Target: Acid Phosphatase (ACP)

Reactivity: Potato

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acid Phosphatase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Product Details anti-Acid Phosphatase Antibody (HRP)

Supplier Product No.: 200-4340-0100

Supplier: Rockland

Purpose: Acid Phosphatase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].

Characteristics: Synonyms: rabbit anti-Acid Phosphatase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Acid Phosphatase [Potato]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Acid Phosphatase Peroxidase Conjugated Antibody has been tested by dot blot and western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Acid Phosphatase

Target: Acid Phosphatase (ACP)

Alternative Name: Acid Phosphatase

Background: Background: The catalytic activity of Acid Phosphatase is phosphate monoester + H2O = an alcohol + phosphate.

UniProt: Q6J5M8