Acid Phosphatase antibody (HRP)
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- Target See all Acid Phosphatase (ACP) Antibodies
- Acid Phosphatase (ACP)
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Reactivity
- Potato
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Acid Phosphatase antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Dot Blot (DB)
- Supplier Product No.
- 200-4340-0100
- Supplier
- Rockland
- Purpose
- Acid Phosphatase Antibody Peroxidase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].
- Characteristics
- Synonyms: rabbit anti-Acid Phosphatase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Acid Phosphatase [Potato]
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product ACP Primary Antibody
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- Application Notes
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Application Note: Anti-Acid Phosphatase Peroxidase Conjugated Antibody has been tested by dot blot and western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:4,000 - 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Acid Phosphatase (ACP)
- Alternative Name
- Acid Phosphatase (ACP Products)
- Background
- Background: The catalytic activity of Acid Phosphatase is phosphate monoester + H2O = an alcohol + phosphate.
- UniProt
- Q6J5M8
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