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COL1A1 antibody (HRP)

This Rabbit Polyclonal antibody specifically detects COL1A1 in WB, IHC, ELISA, IP and FACS. It exhibits reactivity toward Human, Rat, Mouse and Cow.
Rockland
Catalog No. ABIN5596823
Supplier Product No.: 600-403-103

Quick Overview for COL1A1 antibody (HRP) (ABIN5596823)

Target

See all COL1A1 Antibodies
COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivity

  • 69
  • 32
  • 27
  • 12
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Rat, Mouse, Cow

Host

  • 81
  • 17
Rabbit

Clonality

  • 70
  • 28
Polyclonal

Conjugate

  • 60
  • 10
  • 8
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This COL1A1 antibody is conjugated to HRP

Application

  • 69
  • 55
  • 34
  • 27
  • 26
  • 7
  • 6
  • 5
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)
  • Supplier Product No.

    600-403-103

    Supplier

    Rockland

    Purpose

    Collagen Type I Antibody Peroxidase Conjugated

    Cross-Reactivity (Details)

    Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

    Characteristics

    Synonyms: rabbit anti-collagen type I antibody peroxidase conjugation, HRP conjugated rabbit anti-collagen type I antibody, Collagen Of Skin Tendon And Bone, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen alpha-1 (I) chain, Alpha-1 type I collagen, type 1 procollagen alpha 1

    Purification

    This product has been prepared by immunoaffinity chromatography using immobilized antigens.

    Immunogen

    Immunogen: Collagen Type I from human and bovine placenta.

    Immunogen Type: Native Protein

    Isotype

    IgG
  • Application Notes

    Flow Cytometry Dilution: 5 μg/mL

    Immunohistochemistry Dilution: 1:50 - 1:200

    Application Note: Anti-COLLAGEN Type I Peroxidase Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:3,000 - 1:6,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:3,000 - 1:6,000

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Volume: 50μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Thimerosal

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at 4° C prior to restoration.   Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

    Expiry Date

    12 months
  • Target

    COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

    Alternative Name

    COL1A1

    Background

    Background: COLLAGEN Type I Peroxidase Conjugated Antibody is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

    Gene ID

    1277

    NCBI Accession

    NP_000079

    UniProt

    P02452

    Pathways

    Sensory Perception of Sound, Autophagy, Growth Factor Binding
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