Collagen, Type III (COL3) antibody

Details for Product No. ABIN5596830
Antigen
  • PT
  • RPRGL2
  • THPH1
  • Cf-2
  • Cf2
  • FII
  • thrombin
  • prothrombin
  • coagulation factor II, thrombin
  • coagulation factor II
  • F2
Reactivity
Cow (Bovine), Human, Mammalian
141
63
51
15
7
3
1
1
1
Host
Rabbit
123
24
21
Clonality
Polyclonal
Conjugate
Un-conjugated
13
5
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
115
80
73
43
42
34
26
24
19
11
8
7
5
3
2
2
2
1
1
1
1
Options
Immunogen

Immunogen: Collagen Type III from human and bovine placenta

Immunogen Type: Native Protein

Isotype IgG
Cross-Reactivity (Details) Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Purification Collagen III Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Antigen
Alternative Name Collagen Type III (COL3 Antibody Abstract)
Background

Synonyms: Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1

Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

Gene Name: COL3A1

Gene ID 1281
UniProt P02461
Application Notes

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:50,000

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months
Supplier Images
 image for anti-Collagen, Type III (COL3) antibody (ABIN5596830) anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong st...
Western Blotting (WB) image for anti-Collagen, Type III (COL3) antibody (ABIN5596830) Western Blot of Rabbit Anti-COLLAGEN III Antibody Lane 1: Human Collagen III Load: 10...
Immunohistochemistry (IHC) image for anti-Collagen, Type III (COL3) antibody (ABIN5596830) Immunohistochemistry of Rabbit Anti-collagen type III antibody. Tissue: right lobe of...
Product cited in: Andrews, Marttala, Macarak, Rosenbloom, Uitto: "Keloid Pathogenesis: Potential Role of Cellular Fibronectin with the EDA Domain." in: The Journal of investigative dermatology, Vol. 135, Issue 7, pp. 1921-1924, 2015 (PubMed).

Pohjoismäki, Williams, Boettger, Goffart, Kim, Suomalainen, Moraes, Braun: "Overexpression of Twinkle-helicase protects cardiomyocytes from genotoxic stress caused by reactive oxygen species." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 48, pp. 19408-13, 2014 (PubMed).

Olczyk, Wisowski, Komosinska-Vassev, Stojko, Klimek, Olczyk, Kozma: "Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 423809, 2013 (PubMed).

Koźma, Wisowski, Kusz, Olczyk: "The role of decorin and biglycan dermatan sulfate chain(s) in fibrosis-affected fascia." in: Glycobiology, Vol. 21, Issue 10, pp. 1301-16, 2012 (PubMed).

Pinheiro, Ferreira, Kitten, da Silveira, da Silva, Santos, Gava, Castro, Magalhães, da Mota, Botelho-Santos, Bader, Alenina, Santos, Simoes E Silva: "Genetic deletion of the angiotensin-(1-7) receptor Mas leads to glomerular hyperfiltration and microalbuminuria." in: Kidney international, Vol. 75, Issue 11, pp. 1184-1193, 2009 (PubMed).

Did you look for something else?