MEK1 antibody (C-Term) (Biotin)

Catalog No. ABIN5596862

Supplier Product No.: 200-306-gq0

Product Details anti-MEK1 Antibody (Biotin)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEK1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-306-gq0

Supplier: Rockland

Purpose: MEK1 C-Term Antibody Biotin

Cross-Reactivity (Details): This biotin conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK1.

Characteristics: Synonyms: mouse anti-MEK1 antibody biotin conjugation, biotin conjugated mouse anti-MEK1 antibody, MAP2K, MEK, MEK 1, MKK1, PRKMK1, MEK-1 Antibody

Purification: Anti-MEK1 is purified from tissue culture supernatant by protein A purification.

Immunogen:

Immunogen: Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

Immunogen Type: Conjugated Peptide

Clone: 13B6-G12

Isotype: IgG1 kappa

Labeling Ratio: 10-20

Application Details

Application Notes:

Application Note: Anti-MEK 1 Biotin Conjugated (MOUSE) Antibody has been tested by Western Blotting and ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:40,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1 (MAP2K1 Products)

Background: Background: MEK1 antibodies detect the MEK1 isoform. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling