Phosphoserine antibody (HRP)

Catalog No. ABIN5596875

Supplier Product No.: 600-403-261

Product Details anti-Phosphoserine Antibody (HRP)

Target: Phosphoserine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphoserine antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Supplier Product No.: 600-403-261

Supplier: Rockland

Purpose: Phosphoserine Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Phosphoserine antibody detects proteins phosphorylated on serine residues.

Characteristics: Synonyms: Phosphoserine [pS] reactive antibody, Phosphoserine [pS] polyclonal antibody, Phosphoserine antibody produced in rabbit

Purification: Anti-Phosphoserine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Phosphoserine antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: HRP Conjugated Anti-Phosphoserine antibody is suitable for use in ELISA and western blotting assays.  The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues.  The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 40 % (v/v) Glycerol

Preservative: None. Do NOT add sodium azide!

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store pS antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Phosphoserine

Target: Phosphoserine

Abstract: Phosphoserine Products

Target Type: Amino Acid

Background: Background: Anti-Phosphoserine Antibody recognizes phosphoserine, peptidyl-phosphoserine, and serine-phosphorylated proteins