Phosphothreonine antibody (phosphorylated) (HRP)

Catalog No. ABIN5596878

Product Details anti-Phosphothreonine Antibody (HRP)

Target: Phosphothreonine

Binding Specificity: phosphorylated

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphothreonine antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to horseradish peroxidase and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Peroxidase.

Immunogen:

Immunogen: Phospho Site Specific affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Phospho Site Specific Threonine affinity purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine proteins in both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Phosphothreonine antibody (HRP) (ABIN5596878)

Puttagunta, Tedeschi, Sória, Hervera, Lindner, Rathore, Gaub, Joshi, Nguyen, Schmandke, Laskowski, Boutillier, Bradke, Di Giovanni: "PCAF-dependent epigenetic changes promote axonal regeneration in the central nervous system." in: Nature communications, Vol. 5, pp. 3527, (2014) (PubMed).

Target Details for Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Products

Target Type: Amino Acid

Background:

Synonyms: Phospho-Threonine Antibody, Phospho-Threonine ELISA, HRP Phospho Threonine (pT) antibody, PhosphoThreonine (pT) polyclonal antibody

Background: Phospho Site Specific Phosph Threonine antibody is ideal for ELISA where Signal-to-noise ratio of phospho- versus nonphospho-peptides is critical. Phospho-Threonine Antibody (P-Thr-Polyclonal) is also functional by Western Blotting.