Glucose Oxidase antibody (HRP)

Catalog No. ABIN5596886

Supplier Product No.: 200-4337-0100

This Rabbit Polyclonal antibody specifically detects Glucose Oxidase in ELISA, WB and IHC. It exhibits reactivity toward Aspergillus niger.

Quick Overview for Glucose Oxidase antibody (HRP) (ABIN5596886)

Target: Glucose Oxidase (GOD)

Reactivity: Aspergillus niger

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Glucose Oxidase Antibody (HRP)

Supplier Product No.: 200-4337-0100

Supplier: Rockland

Purpose: Glucose Oxidase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].

Characteristics: Synonyms: rabbit anti-Glucose Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Purification: Glucose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glucose Oxidase [Aspergillus niger]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Glucose Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in Immunohistochemistry. Optimal titers should be obtained by researcher.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase

Background: Background: Glucose Oxidase is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites, used for the determination of free glucose in body fluids, and has many applications in biotechnologies.

UniProt: P13006