CA1 antibody (HRP)

Catalog No. ABIN5596907

Supplier Product No.: 200-1354-0100

This Goat Polyclonal antibody specifically detects CA1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CA1 antibody (HRP) (ABIN5596907)

Target: CA1 (Carbonic Anhydrase I (CA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CA1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CA1 Antibody (HRP)

Supplier Product No.: 200-1354-0100

Supplier: Rockland

Purpose: Carbonic Anhydrase I Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes ].

Characteristics: Synonyms: goat anti-Carbonic Anhydrase I Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Carbonic Anhydrase I Antibody, Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

Purification: Carbonic Anhydrase I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Carbonic Anhydrase I [Human Erythrocytes ]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Carbonic Anhydrase I peroxidase is tested by ELISA and western blot and useful in immunohistochemistry. Optimal titers should be obtained by researchers.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Alternative Name: CA1

Background: Background: Carbonic Anhydrase I is an enzyme that in humans is encoded by the CA1 gene. Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including cellular respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.

Gene ID: 759

NCBI Accession: NP_001122301

UniProt: P00915