GAPDH antibody

Catalog No. ABIN559691

The Mouse Monoclonal anti-GAPDH antibody has been validated for WB, ELISA, DB and ISt. It is suitable to detect GAPDH in samples from Human, Mouse, Rat, Rabbit, Chicken, Hamster, Saccharomyces cerevisiae, Insect and Bacteria. There are 3+ publications available.

Quick Overview for GAPDH antibody (ABIN559691)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Rabbit, Chicken, Hamster, Saccharomyces cerevisiae, Insect, Bacteria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Immunostaining (ISt)

Clone: GA1R

Product Details anti-GAPDH Antibody

Specificity: Recognizes native and denatured forms of GAPDH (∼37kDa). GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster / GAPDH from other species may also be detectable

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogen: Recombinant GAPDH

Isotype: IgG1

Application Details

Application Notes: WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-GAPDH antibody (ABIN559691)

Steinbrecht, Pfeifer, Herzog, Katzenberger, Schulz, Kammerer, Küpper: "HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells." in: Toxicology letters, Vol. 319, pp. 155-159, (2019) (PubMed).

Martínez-Moya, Ortega-González, González, Anzola, Ocón, Hernández-Chirlaque, López-Posadas, Suárez, Zarzuelo, Martínez-Augustin, Sánchez de Medina: "Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats." in: Pharmacological research : the official journal of the Italian Pharmacological Society, Vol. 66, Issue 2, pp. 144-53, (2012) (PubMed).

Gandhi, Takahashi, Virtanen, Syed, Davey, Mahomed: "Microarray analysis of the infrapatellar fat pad in knee osteoarthritis: relationship with joint inflammation." in: The Journal of rheumatology, Vol. 38, Issue 9, pp. 1966-72, (2011) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH