Caveolin-1 antibody (N-Term)

Catalog No. ABIN5596911

Supplier Product No.: 600-401-j63

The Rabbit Polyclonal anti-Caveolin-1 antibody has been validated for WB and ELISA. It is suitable to detect Caveolin-1 in samples from Human.

Quick Overview for Caveolin-1 antibody (N-Term) (ABIN5596911)

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caveolin-1 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-j63

Supplier: Rockland

Purpose: Caveolin-1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with lemur, sheep, bat, rat, rabbit, pig, and primate based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-Caveolin-1 Antibody, CAV1, CAV, CAV 1, rabbit anti-Caveolin 1 Antibody

Purification: Anti-Caveolin 1 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Caveolin 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-terminus of human Caveolin-1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Caveolin1 antibody has been tested in WB and is useful for ELISA and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~21 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.78 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: CAV1

Background: Background: Caveolin 1 is a ubiquitous protein found within the membranes of caveolea, theoretically acting as a scaffolding protein. Direct interaction with G-protein alpha subunits allows regulation of their activity. A regulatory protein, it is involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation, and is also a factor in the regulation of CTNNB1-mediated signaling through the Wnt pathway. Expressed in the muscle and lung tissues predominantly, defects in CAV1 is the cause of congenital generalized lipodystrophy type 3, or autosomal recessive disorders such as adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset diabetes. Anti-Caveolin 1 antibody is ideal for researchers in Metabolism, Cancer, Signal Transduction, and Cardiovascular research.

UniProt: Q03135

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals