Trypsin Inhibitor antibody (HRP)

Catalog No. ABIN5596918

Supplier Product No.: 200-4387-0100

The HRP-conjugated Rabbit Polyclonal anti-Trypsin Inhibitor antibody (ABIN5596918) specifically detects Trypsin Inhibitor in WB, IP and DB. The antibody is reactive with Lima bean samples.

Quick Overview for Trypsin Inhibitor antibody (HRP) (ABIN5596918)

Target: Trypsin Inhibitor

Reactivity: Lima bean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Trypsin Inhibitor antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Product Details anti-Trypsin Inhibitor Antibody (HRP)

Supplier Product No.: 200-4387-0100

Supplier: Rockland

Purpose: Trypsin Inhibitor Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].

Characteristics: Synonyms: rabbit anti-trypsin inhibitor antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Purification: Trypsin Inhibitor is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Trypsin Inhibitor [Lima Bean]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Trypsin Inhibitor Peroxidase Conjugated Antibody has been tested by Dot blot and is suitable to be assayed against 1.0 μg of Trypsin Inhibitor [Lima Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250

Immunoprecipitation Dilution: 1:100

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Trypsin Inhibitor

Target: Trypsin Inhibitor

Background: Background: Trypsin Inhibitors are chemicals that reduce the availability of biologically active trypsin, an enzyme essential to nutrition of many animals, including humans. Tumor Associated Trypsin Inhibitor (TATI) has been used as a marker for various carcinomas and useful in cancer diagnosis.

Gene ID: 547831

UniProt: P01070