KMO antibody (N-Term)

Catalog No. ABIN5596944

Supplier Product No.: 600-401-j84

The Rabbit Polyclonal anti-KMO antibody has been validated for WB and ELISA. It is suitable to detect KMO in samples from Mouse.

Quick Overview for KMO antibody (N-Term) (ABIN5596944)

Target: KMO (Kynurenine 3-Monooxygenase (Kynurenine 3-Hydroxylase) (KMO))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KMO antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-KMO Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-j84

Supplier: Rockland

Purpose: Kmo Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with mouse and rat based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-Kmo antibody, kynurenine 3-monooxygenase, kynurenine 3-hydroxylase

Purification: Anti-KMO was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-KMO affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide at the N-terminal of mouse KMO protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-KMO antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~58 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.01 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for KMO

Target: KMO (Kynurenine 3-Monooxygenase (Kynurenine 3-Hydroxylase) (KMO))

Alternative Name: Kmo

Background: Background: Kynurenine 3-monooxygenase (KMO) catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). The enzyme is required for synthesis of quinolinic acid. Quinolinic acid is a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Anti-KMO antibodies are ideal for researchers interested in Apoptosis, Neurodegeneration, and Neuroscience research.