R-Phycoerythrin antibody

Catalog No. ABIN5596962

Supplier Product No.: 600-101-387

The Goat Polyclonal anti-R-Phycoerythrin antibody has been validated for WB, ELISA and IHC. It is suitable to detect R-Phycoerythrin in samples from . There is 1 publication available.

Quick Overview for R-Phycoerythrin antibody (ABIN5596962)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Host: Goat

Clonality: Polyclonal

Conjugate: This R-Phycoerythrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-R-Phycoerythrin Antibody

Supplier Product No.: 600-101-387

Supplier: Rockland

Purpose: R-Phycoerythrin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.

Characteristics: Synonyms: goat anti R-Phycoerythrin Antibody, goat anti-R-Phycoerythrin Antibody, goat anti Phycoerythrin

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-R-Phycoerythrin Antibody is tested by ELISA and suitable for western blotting, fluorescent assays, immunoprecipitation, immunodiffusion, conjugation to magnetic particles (beads) and most other immunological methods requiring lot-to-lot consistency, high titer and specificity.  Although not specifically tested, this reagent should also be useful for FACS and other fluorescent immunoassays.  R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.  Reaction with low molecular fragments is typically noted by western blot.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-R-Phycoerythrin antibody (ABIN5596962)

Veenstra, Liu, Lee, Du, Tang, Kern: "Diabetic Retinopathy: Retina-Specific Methods for Maintenance of Diabetic Rodents and Evaluation of Vascular Histopathology and Molecular Abnormalities." in: Current protocols in mouse biology, Vol. 5, Issue 3, pp. 247-270, (2016) (PubMed).

Target Details for R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Alternative Name: R-Phycoerythrin

Target Type: Chemical

Background: Background: Anti-R-Phycoerythrin Antibody generated in goat recognizes RPE. Phycoerythrin (PE) is a red protein-pigment present in red algae and cryptophytes and an accessory pigment to the main chlorophyll pigments responsible for photosynthesis. R-phycoerythrin is predominantly produced by red algae. The molecular weight of R-PE is 240 kDa. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

UniProt: Q7SIG0