R-Phycoerythrin antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN5596963

Supplier Product No.: 600-105-387

This anti-R-Phycoerythrin antibody is a Goat Polyclonal antibody detecting R-Phycoerythrin in WB, ELISA and IHC. Suitable for . This Primary Antibody has been cited in 1 publication.

Quick Overview for R-Phycoerythrin antibody (Alkaline Phosphatase (AP)) (ABIN5596963)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Host: Goat

Clonality: Polyclonal

Conjugate: This R-Phycoerythrin antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-R-Phycoerythrin Antibody (Alkaline Phosphatase (AP))

Supplier Product No.: 600-105-387

Supplier: Rockland

Purpose: R-Phycoerythrin Antibody Alkaline Phosphatase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, and R-Phycoerythrin conjugated IgG.

Characteristics: Synonyms: goat anti-R-Phycoerythrin Antibody Alkaline Phosphatase Conjugation, Alk phos conjugated goat anti-R-Phycoerythrin Antibody

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.  R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.  Reaction with low molecular fragments is typically noted by western blot.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

References for anti-R-Phycoerythrin antibody (Alkaline Phosphatase (AP)) (ABIN5596963)

Beute, Lukkes, Koekoek, Nastiti, Ganesh, de Bruijn, Hockman, van Nimwegen, Braunstahl, Boon, Lambrecht, Manganiello, Hendriks, KleinJan: "A pathophysiological role of PDE3 in allergic airway inflammation." in: JCI insight, Vol. 3, Issue 2, (2018) (PubMed).

Target Details for R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Alternative Name: R-Phycoerythrin

Target Type: Chemical

Background: Background: Anti-R-Phycoerythrin Antibody Alkaline Phosphatase Conjugated generated in goat recognizes RPE. Phycoerythrin (PE) is a red protein-pigment present in red algae and cryptophytes and an accessory pigment to the main chlorophyll pigments responsible for photosynthesis. R-phycoerythrin is predominantly produced by red algae. The molecular weight of R-PE is 240 kDa. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-R-PE antibody is conjugated to Alk Phos.