Tropomyosin antibody (pSer283)

Catalog No. ABIN5596982

Supplier Product No.: 600-401-j52

Product Details anti-Tropomyosin Antibody

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Binding Specificity: pSer283

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tropomyosin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-401-j52

Supplier: Rockland

Purpose: Tropomyosin-1 alpha phospho S283 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-Tropomyosin-1 alpha pS283 antibody, rabbit anti-Tropomyosin1 alpha pS283 antibody, Tropomyosin α-1 chain, Alpha-tropomyosin, α-tropomyosin, Tropomyosin-1, TPM1, TPM-1, C15orf13, TMSA

Purification: Anti-Tropomyosin-1 alpha pS283 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human S283 residue.

Immunogen:

Immunogen: Tropomyosin-1 alpha pS283 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding S283 human Tropomyosin-1 alpha pS283.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Tropomyosin-1 alpha pS283 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~32.7 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 50μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tropomyosin

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Alternative Name: TPM1 (TPM1 Products)

Background: Background: Anti-Tropomyosin-1 alpha pS283 antibody attaches to actin filaments in muscle and non-muscle cells, a large factor in the calcium dependent regulation of muscle contraction. Anti-Tropomyosin-1 alpha pS283 antibody is ideal for researchers interested in Signal Transduction and Cardiovascular research.

Gene ID: 7168

NCBI Accession: NP_000357

UniProt: P09493

Pathways: Regulation of Actin Filament Polymerization