SIRPA antibody (AA 235-373)

Catalog No. ABIN5611267

This anti-SIRPA antibody is a Mouse Monoclonal antibody detecting SIRPA in FACS and ELISA. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for SIRPA antibody (AA 235-373) (ABIN5611267)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA

Clone: 2H7E2

Product Details anti-SIRPA Antibody

Binding Specificity: AA 235-373

Purpose: CD172A Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human CD172A (AA: 235-373) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SIRPA antibody (ABIN5611267)

Deuse, Hu, Agbor-Enoh, Jang, Alawi, Saygi, Gravina, Tediashvili, Nguyen, Liu, Valantine, Lanier, Schrepfer: "The SIRPα-CD47 immune checkpoint in NK cells." in: The Journal of experimental medicine, Vol. 218, Issue 3, (2021) (PubMed).

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172A

Background: The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.

Molecular Weight: 55 kDa

UniProt: P78324