beta Actin antibody (N-Term)

Catalog No. ABIN5611427

The Rabbit Polyclonal anti-beta Actin antibody has been validated for WB and IHC (p). It is suitable to detect beta Actin in samples from Human, Mouse, Rat, Chicken, Rabbit, Dog, Pig, Cow, Sheep, Guinea Pig and Cat.

Quick Overview for beta Actin antibody (N-Term) (ABIN5611427)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Pig, Cow, Sheep, Guinea Pig, Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Specificity: This antibody recognizes Actin-Beta (ACTB).

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Cat, Chicken, Canine (Dog), Guinea Pig, Mouse, Porcine, Rabbit, Rat and Sheep.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: N-terminal Human beta Actin.

Application Details

Application Notes: Western blot. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by Alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 10 mM PBS, pH 7.4 containing BSA and Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Actin beta / ACTB

Background: Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. There are three alpha-actins (skeletal, cardiac, and smooth muscle), one beta actin (non-muscle), and two gamma-actins (gamma-smooth muscle and gamma-nonmuscle). Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.Synonyms: Actin cytoplasmic 1, Beta-Actin

Gene ID: 60

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction