CNOT3 antibody (AA 1-100)

Catalog No. ABIN562024

This Mouse Monoclonal antibody specifically detects CNOT3 in WB, ELISA and IF. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for CNOT3 antibody (AA 1-100) (ABIN562024)

Target: CNOT3 (CCR4-NOT Transcription Complex, Subunit 3 (CNOT3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNOT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 4B8

Product Details anti-CNOT3 Antibody

Binding Specificity: AA 1-100

Purpose: Mouse monoclonal antibody raised against a partial recombinant CNOT3.

Sequence: MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: CNOT3 (NP_055331, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-CNOT3 antibody (ABIN562024)

Inoue, Morita, Hijikata, Fukuda-Yuzawa, Adachi, Isono, Ikawa, Kawamoto, Koseki, Natsume, Fukao, Ohara, Yamamoto, Kurosaki: "CNOT3 contributes to early B cell development by controlling Igh rearrangement and p53 mRNA stability." in: The Journal of experimental medicine, Vol. 212, Issue 9, pp. 1465-79, (2015) (PubMed).

Kamon, Katano, Hiraki-Kamon, Hishida, Nakachi, Mizuno, Okazaki, Suzuki, Hirasaki, Ueda, Nishimoto, Kato, Okuda: "Identification of Ccr4-not complex components as regulators of transition from partial to genuine induced pluripotent stem cells." in: Stem cells and development, Vol. 23, Issue 18, pp. 2170-9, (2014) (PubMed).

Doidge, Mittal, Aslam, Winkler: "The anti-proliferative activity of BTG/TOB proteins is mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) deadenylase subunits of the Ccr4-not complex." in: PLoS ONE, Vol. 7, Issue 12, pp. e51331, (2012) (PubMed).

Aslam, Mittal, Koch, Andrau, Winkler: "The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation." in: Molecular biology of the cell, Vol. 20, Issue 17, pp. 3840-50, (2009) (PubMed).

Target Details for CNOT3

Target: CNOT3 (CCR4-NOT Transcription Complex, Subunit 3 (CNOT3))

Alternative Name: CNOT3

Background: Full Gene Name: CCR4-NOT transcription complex, subunit 3
Synonyms: KIAA0691,LENG2,NOT3,NOT3H

Gene ID: 4849

NCBI Accession: NM_014516

Pathways: Stem Cell Maintenance