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PITX1 antibody (AA 225-313)

Name: PITX1 antibody (AA 225-313)
SKU: ABIN562248
Price: 781.72 USD
Availability: InStock

PITX1 Reactivity: Human WB, ELISA, IP, RNAi Host: Mouse Monoclonal 5G4 unconjugated

PITX1 monoclonal antibody (M01), clone 5G4 Western Blot analysis of PITX1 expression in Hela S3 NE . (PITX1 antibody (AA 225-313))

Detection limit for recombinant GST tagged PITX1 is approximately 0. (PITX1 antibody (AA 225-313))

ELISA

Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). (PITX1 antibody (AA 225-313))

Immunoprecipitation of PITX1 transfected lysate using anti-PITX1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PITX1 MaxPab rabbit polyclonal antibody. (PITX1 antibody (AA 225-313))

Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4. (PITX1 antibody (AA 225-313))

Western Blot detection against Immunogen (35. (PITX1 antibody (AA 225-313))

6 images

(2 references)

Catalog No. ABIN562248

Datasheet as PDF

Target See all PITX1 Antibodies

PITX1 (Paired-Like Homeodomain 1 (PITX1))
Binding Specificity
4

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 225-313
Reactivity
30

20

20

6

6

5

4

3

3

3

2

1
Human
Host
29

2
Mouse
Compatible Secondaries
Clonality
30

1
Monoclonal
Conjugate
24

1

1

1

1

1

1

1
This PITX1 antibody is un-conjugated
Application
24

9

8

4

3

3

2

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), RNA Interference (RNAi)

Purpose

Mouse monoclonal antibody raised against a partial recombinant PITX1.

Sequence

MPSSMGPGAV PGMPNSGLNN INNLTGSSLN SAMSPGACPY GTPASPYSVY RDTCNSSLAS LRLKSKQHSS FGYGGLQGPA SGLNACQYN

Cross-Reactivity

Human

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

PITX1 (NP_002644, 225 a.a. ~ 313 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

5G4

Isotype

IgG2a
anti-Paired-Like Homeodomain 1 (PITX1) (AA 1-314) antibody
PITX1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) (AA 123-150) antibody
PITX1 Reactivity: Human WB, ELISA, IHC, FACS Host: Rabbit Polyclonal unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) (AA 123-150) antibody
PITX1 Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB18913 unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) (N-Term) antibody
PITX1 Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Dog WB Host: Rabbit Polyclonal unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) (Internal Region) antibody
PITX1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) (Internal Region) antibody
PITX1 Reactivity: Human, Rat, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) (Internal Region) antibody
PITX1 Reactivity: Human, Rat, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) antibody
PITX1 Reactivity: Rat WB Host: Rabbit Polyclonal unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) (C-Term) antibody
PITX1 Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Zebrafish (Danio rerio), Pig WB Host: Rabbit Polyclonal unconjugated

anti-Paired-Like Homeodomain 1 (PITX1) (AA 160-209) antibody
PITX1 Reactivity: Human, Rat, Mouse, Guinea Pig, Dog, Horse, Pig, Monkey, Bat WB Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Yamaguchi, Miki, Yoshida: "The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage." in: Apoptosis : an international journal on programmed cell death, Vol. 15, Issue 8, pp. 927-35, (2010) (PubMed).

Liu, Lobie: "Transcriptional activation of p53 by Pitx1." in: Cell death and differentiation, Vol. 14, Issue 11, pp. 1893-907, (2007) (PubMed).
Target

PITX1 (Paired-Like Homeodomain 1 (PITX1))

Alternative Name

PITX1 (PITX1 Products)

Background

Full Gene Name: paired-like homeodomain 1
Synonyms: BFT,POTX,PTX1

Gene ID

5307

NCBI Accession

NM_002653