Topoisomerase I antibody (AA 692-765)

Catalog No. ABIN563222

The Mouse Monoclonal anti-Topoisomerase I antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect Topoisomerase I in samples from Human. There is 1 publication available.

Quick Overview for Topoisomerase I antibody (AA 692-765) (ABIN563222)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1A1

Product Details anti-Topoisomerase I Antibody

Binding Specificity: AA 692-765

Purpose: Mouse monoclonal antibody raised against a partial recombinant TOP1.

Sequence: QRLEEQLMKL EVQATDREEN KQIALGTSKL NYLDPRITVA WCKKWGVPIE KIYNKTQREK FAWAIDMADE DYEF

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: TOP1 (NP_003277, 692 a.a. ~ 765 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Topoisomerase I antibody (ABIN563222)

Kobayashi, Aida, Nagaoka, Begum, Kitawaki, Nakata, Stanlie, Doi, Kato, Okazaki, Shinkura, Muramatsu, Kinoshita, Honjo: "AID-induced decrease in topoisomerase 1 induces DNA structural alteration and DNA cleavage for class switch recombination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 52, pp. 22375-80, (2010) (PubMed).

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1

Background: Full Gene Name: topoisomerase (DNA) I
Synonyms: TOPI

Gene ID: 7150

NCBI Accession: NM_003286

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance