Syndecan 1 antibody (PE)

Catalog No. ABIN5633266

The Mouse Monoclonal anti-Syndecan 1 antibody has been validated for FACS. It is suitable to detect Syndecan 1 in samples from Human and Non-Human Primate.

Quick Overview for Syndecan 1 antibody (PE) (ABIN5633266)

Target: Syndecan 1 (SDC1)

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Syndecan 1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: MI15

Product Details anti-Syndecan 1 Antibody (PE)

Purpose: Anti-Hu CD138 PE

Specificity: The mouse monoclonal antibody MI15 recognizes an extracellular epitope of CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.

Characteristics: The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: A mixture of U266 and XG-1 human myeloma cell lines

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for Syndecan 1

Target: Syndecan 1 (SDC1)

Alternative Name: CD138

Background: CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism