CD5 antibody (PE)

Catalog No. ABIN5633275

The Rat Monoclonal anti-CD5 antibody has been validated for FACS. It is suitable to detect CD5 in samples from Mouse.

Quick Overview for CD5 antibody (PE) (ABIN5633275)

Target: CD5

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 53-7-3

Product Details anti-CD5 Antibody (PE)

Purpose: Anti-Ms CD5 PE

Specificity: The rat monoclonal antibody 53-7.3 recognizes an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B-1 lymphocytes.

Cross-Reactivity (Details): Mouse

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: murine thymus or spleen cells

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1

Gene ID: 12507

UniProt: P13379