Recombinant CD79a antibody (AA 202-216)

Catalog No. ABIN5646923

This anti-CD79a antibody is a Rabbit Monoclonal antibody detecting CD79a in FACS, IF, IHC (p). Suitable for Human, Mouse, Rat, Cow, Pig, Monkey.

Quick Overview for Recombinant CD79a antibody (AA 202-216) (ABIN5646923)

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD79a antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IGA-1790R

Product Details anti-CD79a Antibody

Binding Specificity: AA 202-216

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for the recombinant CD79a antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant CD79a antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,IHC (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CD79a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Alternative Name: CD79a

Background: A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

Pathways: BCR Signaling