Recombinant gamma H2AX antibody (pSer139)

Catalog No. ABIN5647042

The Rabbit Monoclonal anti-gamma H2AX antibody has been validated for WB, ICC and ELISA. It is suitable to detect gamma H2AX in samples from All Species.

Quick Overview for Recombinant gamma H2AX antibody (pSer139) (ABIN5647042)

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Antibody Type: Recombinant Antibody

Reactivity: All Species

Host: Rabbit

Clonality: Monoclonal

Conjugate: This gamma H2AX antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA

Clone: RM224

Product Details anti-gamma H2AX Antibody

Binding Specificity: pSer139

Characteristics: This recombinant Gamma H2AX antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

Purification: Purified

Immunogen: A phospho-peptide corresponding to phospho-Histone H2AX (S139) was used as the immunogen for this recombinant Gamma H2AX antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Gamma H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant Gamma H2AX antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for gamma H2AX

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Alternative Name: Gamma H2AX

Background: This recombinant Gamma H2AX antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

Gene ID: 3014

UniProt: P16104