LC3B antibody (pThr93, pTyr99)

Catalog No. ABIN3028838

The Rabbit Polyclonal anti-LC3B antibody has been validated for ELISA and DB. It is suitable to detect LC3B in samples from Human.

Quick Overview for LC3B antibody (pThr93, pTyr99) (ABIN3028838)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: ELISA, Dot Blot (DB)

Product Details anti-LC3B Antibody

Binding Specificity: pThr93, pTyr99

Purification: Antigen affinity purified

Immunogen: This phospho-LC3B antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT93/Y99 of human MAP1LC3B.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-LC3B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: LC3B

Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

UniProt: Q9GZQ8

Pathways: Autophagy