VASP antibody (AA 78-114)

Catalog No. ABIN5647624

The Rabbit Polyclonal anti-VASP antibody is suitable to detect VASP in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for VASP antibody (AA 78-114) (ABIN5647624)

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VASP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-VASP Antibody

Binding Specificity: AA 78-114

Purification: Antigen affinity purified

Immunogen: Amino acids 78-114 (NFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALE-human) were used as the immunogen for this VASP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the VASP antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the VASP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Alternative Name: VASP

Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation