ERK1/2 antibody (pThr202, pTyr204)

Catalog No. ABIN3031824

This anti-ERK1/2 antibody is a Rabbit Polyclonal antibody detecting ERK1/2 in WB, ELISA and DB. Suitable for Human and Mouse.

Quick Overview for ERK1/2 antibody (pThr202, pTyr204) (ABIN3031824)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Product Details anti-ERK1/2 Antibody

Binding Specificity: pThr202, pTyr204

Cross-Reactivity (Details): Expected species reactivity: Rat, Drosophila

Purification: Antigen affinity purified

Immunogen: This phospho-ERK1/2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT202/pY204 of human ERK1/2.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the phospho-ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500,Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-ERK1/2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: ERK1/2

Background: MAPK3/ERK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.

UniProt: P27361