WWP1 antibody (AA 152-260)

Catalog No. ABIN564794

The Mouse Monoclonal anti-WWP1 antibody has been validated for WB, ELISA, IP, IF, IHC (p). It is suitable to detect WWP1 in samples from Human. There are 6+ publications available.

Quick Overview for WWP1 antibody (AA 152-260) (ABIN564794)

Target: WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WWP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1A7

Product Details anti-WWP1 Antibody

Binding Specificity: AA 152-260

Purpose: Mouse monoclonal antibody raised against a partial recombinant WWP1.

Sequence: CSSSPTIEIQ ENGDALHENG EPSARTTARL AVEGTNGIDN HVPTSTLVQN SCCSYVVNGD NTPSSPSQVA ARPKNTPAPK PLASEPADDT VNGESSSFAP TDNASVTGT

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: WWP1 (NP_008944, 152 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-WWP1 antibody (ABIN564794)

Basheer, Harris, Mentrup, Abreha, Thames, Lea, Swing, Copeland, Jenkins, Price, Matesic: "Cardiomyocyte-specific overexpression of the ubiquitin ligase Wwp1 contributes to reduction in Connexin 43 and arrhythmogenesis." in: Journal of molecular and cellular cardiology, Vol. 88, pp. 1-13, (2015) (PubMed).

Cheng, Cao, Yuan, Li, Tong: "Knockdown of WWP1 inhibits growth and induces apoptosis in hepatoma carcinoma cells through the activation of caspase3 and p53." in: Biochemical and biophysical research communications, Vol. 448, Issue 3, pp. 248-54, (2014) (PubMed).

Chaudhary, Maddika: "WWP2-WWP1 ubiquitin ligase complex coordinated by PPM1G maintains the balance between cellular p73 and ?Np73 levels." in: Molecular and cellular biology, Vol. 34, Issue 19, pp. 3754-64, (2014) (PubMed).

Cao, Xue, Han, Ma, Chen, Tong: "WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) delays cellular senescence by promoting p27(Kip1) degradation in human diploid fibroblasts." in: The Journal of biological chemistry, Vol. 286, Issue 38, pp. 33447-56, (2011) (PubMed).

Peschiaroli, Scialpi, Bernassola, El Sherbini, Melino: "The E3 ubiquitin ligase WWP1 regulates ?Np63-dependent transcription through Lys63 linkages." in: Biochemical and biophysical research communications, Vol. 402, Issue 2, pp. 425-30, (2010) (PubMed).

Edwards, Clowes, Tsang, Connell, Sanderson, Luzio, Reid: "Endogenous spartin (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5." in: The Biochemical journal, Vol. 423, Issue 1, pp. 31-9, (2009) (PubMed).

Target Details for WWP1

Target: WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1))

Alternative Name: WWP1

Background: Full Gene Name: WW domain containing E3 ubiquitin protein ligase 1
Synonyms: AIP5,DKFZp434D2111,Tiul1,hSDRP1

Gene ID: 11059

NCBI Accession: NM_007013