TCF4 antibody (AA 500-600) (Atto 594)

Catalog No. ABIN5650923

The Rabbit Polyclonal anti-TCF4 antibody has been validated for WB and IHC. It is suitable to detect TCF4 in samples from Human.

Quick Overview for TCF4 antibody (AA 500-600) (Atto 594) (ABIN5650923)

Target: TCF4 (Transcription Factor 4 (TCF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF4 antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TCF4 Antibody (Atto 594)

Binding Specificity: AA 500-600

Specificity: Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.,Detects 71 kDa, 45 kDa. 45 kDa band represents a different isoform .

Cross-Reactivity: Human, Mouse

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the C-terminal of Human TCF4 (500-600 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5650923 was sufficient for detection of TCF4 in 15 μg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Alternative Name: TCF4

Gene ID: 6925

NCBI Accession: NP_001077431

UniProt: P15884

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus