AGO2 antibody (AA 1-377)

Catalog No. ABIN565346

The Mouse Monoclonal anti-AGO2 antibody has been validated for WB, ELISA, IF, IHC (p) and RNAi. It is suitable to detect AGO2 in samples from Human. There are 44+ publications available.

Quick Overview for AGO2 antibody (AA 1-377) (ABIN565346)

Target: AGO2 (Argonaute 2 (AGO2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AGO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Clone: 2E12-1C9

Product Details anti-AGO2 Antibody

Binding Specificity: AA 1-377

Purpose: Mouse monoclonal antibody raised against a full length recombinant EIF2C2.

Sequence: MPIQGQPCFC KYAQGADSVE PMFRHLKNTY AGLQLVVVIL PGKTPVYAEV KRVGDTVLGM ATQCVQMKNV QRTTPQTLSN LCLKINVKLG GVNNILLPQG RPPVFQQPVI FLGADVTHPP AGDGKKPSIA AVVGSMDAHP NRYCATVRVQ QHRQEIIQDL AAMVRELLIQ FYKSTRFKPT RIIFYRDGVS EGQFQQVLHH ELLAIREACI KLEKDYQPGI TFIVVQKRHH TRLFCTDKNE RVGKSGNIPA GTTVDTKITH PTEFDFYLCS HAGIQGTSRP SHYHVLWDDN RFSSDELQIL TYQLCHTYVR CTRSVSIPAP AYYAHLVAFR ARYHLVDKEH DSAEGSHTSG QSNGRDHQAL AKAVQVHQDT LRTMYFA

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: EIF2C2 (AAH07633.1, 483 a.a. ~ 859 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-AGO2 antibody (ABIN565346)

Mantel, Hjelmqvist, Walch, Kharoubi-Hess, Nilsson, Ravel, Ribeiro, Grüring, Ma, Padmanabhan, Trachtenberg, Ankarklev, Brancucci, Huttenhower, Duraisingh, Ghiran, Kuo, Filgueira, Martinelli, Marti: "Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria." in: Nature communications, Vol. 7, pp. 12727, (2016) (PubMed).

Wang, Ye, Wei, Han, He, Chen, Chen: "LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner." in: Journal of hematology & oncology, Vol. 9, Issue 1, pp. 117, (2016) (PubMed).

Xiao, Wang, Ni, Li, Zhang, Liu, Pu, Xu, Gao, Zhao, Mu, Wang, Sun, Du, Xu, Zhang, Hiscox, Goodfellow, Zhou: "MicroRNA miR-24-3p Promotes Porcine Reproductive and Respiratory Syndrome Virus Replication through Suppression of Heme Oxygenase-1 Expression." in: Journal of virology, Vol. 89, Issue 8, pp. 4494-503, (2015) (PubMed).

Zhang, Guo, Gao, Huang, Li, Jia, Liu, Feng: "MicroRNA-23 inhibits PRRSV replication by directly targeting PRRSV RNA and possibly by upregulating type I interferons." in: Virology, Vol. 450-451, pp. 182-95, (2014) (PubMed).

Nakanishi, Taccioli, Palatini, Fernandez-Cymering, Cui, Kim, Volinia, Croce: "Loss of miR-125b-1 contributes to head and neck cancer development by dysregulating TACSTD2 and MAPK pathway." in: Oncogene, Vol. 33, Issue 6, pp. 702-12, (2014) (PubMed).

Sasaki, Gross, Xing, Goshima, Bassell: "Identification of axon-enriched microRNAs localized to growth cones of cortical neurons." in: Developmental neurobiology, Vol. 74, Issue 3, pp. 397-406, (2014) (PubMed).

Pai, Siripornmongcolchai, Berentsen, Pakzad, Vieuille, Pallesen, Pajak, Simpson, Armstrong, Wibrand, Bramham: "NMDA receptor-dependent regulation of miRNA expression and association with Argonaute during LTP in vivo." in: Frontiers in cellular neuroscience, Vol. 7, pp. 285, (2014) (PubMed).

Le Guillou, Marthey, Laloë, Laubier, Mobuchon, Leroux, Le Provost: "Characterisation and comparison of lactating mouse and bovine mammary gland miRNomes." in: PLoS ONE, Vol. 9, Issue 3, pp. e91938, (2014) (PubMed).

Zhang, Zuo, Yang, Li, Xue, Zhou, Huang, Zhao, Zhou, Yan, Zhang, Guo, Sun, Guo, Zhang, Fu: "MicroRNA directly enhances mitochondrial translation during muscle differentiation." in: Cell, Vol. 158, Issue 3, pp. 607-19, (2014) (PubMed).

Kamel, Segerman, Punga, Akusjärvi: "Small RNA sequence analysis of adenovirus VA RNA-derived miRNAs reveals an unexpected serotype-specific difference in structure and abundance." in: PLoS ONE, Vol. 9, Issue 8, pp. e105746, (2014) (PubMed).

Llobet-Navas, Rodriguez-Barrueco, de la Iglesia-Vicente, Olivan, Castro, Saucedo-Cuevas, Marshall, Putcha, Castillo-Martin, Bardot, Ezhkova, Iavarone, Cordon-Cardo, Silva: "The microRNA 424/503 cluster reduces CDC25A expression during cell cycle arrest imposed by transforming growth factor ? in mammary epithelial cells." in: Molecular and cellular biology, Vol. 34, Issue 23, pp. 4216-31, (2014) (PubMed).

Xue, Ouyang, Huang, Zhou, Ouyang, Li, Wang, Wu, Wei, Bi, Jiang, Cai, Sun, Zhang, Zhang, Chen, Fu: "Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits." in: Cell, Vol. 152, Issue 1-2, pp. 82-96, (2013) (PubMed).

Wei, Nazari-Jahantigh, Chan, Zhu, Heyll, Corbalán-Campos, Hartmann, Thiemann, Weber, Schober: "The microRNA-342-5p fosters inflammatory macrophage activation through an Akt1- and microRNA-155-dependent pathway during atherosclerosis." in: Circulation, Vol. 127, Issue 15, pp. 1609-19, (2013) (PubMed).

Laffont, Corduan, Plé, Duchez, Cloutier, Boilard, Provost: "Activated platelets can deliver mRNA regulatory Ago2•microRNA complexes to endothelial cells via microparticles." in: Blood, Vol. 122, Issue 2, pp. 253-61, (2013) (PubMed).

Zheng, Zhang, Zhang, Wang, Ye, Xi, Du, Liang: "Single modification at position 14 of siRNA strand abolishes its gene-silencing activity by decreasing both RISC loading and target degradation." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 10, pp. 4017-26, (2013) (PubMed).

Curtale, Mirolo, Renzi, Rossato, Bazzoni, Locati: "Negative regulation of Toll-like receptor 4 signaling by IL-10-dependent microRNA-146b." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 28, pp. 11499-504, (2013) (PubMed).

Karginov, Hannon: "Remodeling of Ago2-mRNA interactions upon cellular stress reflects miRNA complementarity and correlates with altered translation rates." in: Genes & development, Vol. 27, Issue 14, pp. 1624-32, (2013) (PubMed).

Fan, Krutilina, Sun, Sethuraman, Yang, Wu, Yue, Pfeffer: "Comprehensive analysis of microRNA (miRNA) targets in breast cancer cells." in: The Journal of biological chemistry, Vol. 288, Issue 38, pp. 27480-93, (2013) (PubMed).

Martinez-Sanchez, Dudek, Murphy: "Regulation of human chondrocyte function through direct inhibition of cartilage master regulator SOX9 by microRNA-145 (miRNA-145)." in: The Journal of biological chemistry, Vol. 287, Issue 2, pp. 916-24, (2012) (PubMed).

Chiang, Sung, Rice: "Regulation of cyclin T1 and HIV-1 Replication by microRNAs in resting CD4+ T lymphocytes." in: Journal of virology, Vol. 86, Issue 6, pp. 3244-52, (2012) (PubMed).

Target Details for AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Alternative Name: EIF2C2

Background: Full Gene Name: eukaryotic translation initiation factor 2C, 2
Synonyms: AGO2,MGC3183,Q10

Gene ID: 27161

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization