TRIB2 antibody (AA 254-343)

Catalog No. ABIN565396

This Mouse Monoclonal antibody specifically detects TRIB2 in WB, ELISA, IHC (p) and RNAi. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for TRIB2 antibody (AA 254-343) (ABIN565396)

Target: TRIB2 (Tribbles Pseudokinase 2 (TRIB2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi)

Clone: 1B1

Product Details anti-TRIB2 Antibody

Binding Specificity: AA 254-343

Purpose: Mouse monoclonal antibody raised against a partial recombinant TRIB2.

Sequence: FHDIEPSSLF SKIRRGQFNI PETLSPKAKC LIRSILRREP SERLTSQEIL DHPWFSTDFS VSNSAYGAKE VSDQLVPDVN MEENLDPFFN

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: TRIB2 (NP_067675, 254 a.a. ~ 343 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-TRIB2 antibody (ABIN565396)

Xu, Tong, Huang, Zhang, Qiao, Weng, Liu, Wang, Sun: "TRIB2 inhibits Wnt/?-Catenin/TCF4 signaling through its associated ubiquitin E3 ligases, ?-TrCP, COP1 and Smurf1, in liver cancer cells." in: FEBS letters, Vol. 588, Issue 23, pp. 4334-41, (2014) (PubMed).

Wang, Zhang, Weng, Qiao, Ma, Xiao, Yu, Pan, Sun et al.: "Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in ..." in: The Journal of biological chemistry, Vol. 288, Issue 47, pp. 33667-81, (2013) (PubMed).

Qiao, Zhang, Wang: "Ubiquitin E3 ligase SCF(?-TRCP) regulates TRIB2 stability in liver cancer cells." in: Biochemical and biophysical research communications, Vol. 441, Issue 3, pp. 555-9, (2013) (PubMed).

Target Details for TRIB2

Target: TRIB2 (Tribbles Pseudokinase 2 (TRIB2))

Alternative Name: TRIB2

Background: Full Gene Name: tribbles homolog 2 (Drosophila)
Synonyms: C5FW,GS3955,TRB2

Gene ID: 28951

NCBI Accession: NM_021643