PIK3R4 antibody (AA 1259-1358)

Catalog No. ABIN565478

There are 2+ publications for this product available. The Mouse Monoclonal anti-PIK3R4 antibody is suitable to detect PIK3R4 in samples from Human. It has been validated for ELISA, WB, IF and IHC (p).

Quick Overview for PIK3R4 antibody (AA 1259-1358) (ABIN565478)

Target: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIK3R4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1B5

Product Details anti-PIK3R4 Antibody

Binding Specificity: AA 1259-1358

Purpose: Mouse monoclonal antibody raised against a partial recombinant PIK3R4.

Sequence: AGSDMKIRFW DLAYPERSYV VAGSTSSPSV SYYRKIIEGT EVVQEIQNKQ KVGPSDDTPR RGPESLPVGH HDIITDVATF QTTQGFIVTA SRDGIVKVWK

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: PIK3R4 (NP_055417, 1259 a.a. ~ 1358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-PIK3R4 antibody (ABIN565478)

Hirsch, Shen, Dokmanovic, Yu, Mohan, Elzarrad, Wu: "Insulin activation of vacuolar protein sorting 34 mediates localized phosphatidylinositol 3-phosphate production at lamellipodia and activation of mTOR/S6K1." in: Cellular signalling, Vol. 26, Issue 6, pp. 1258-68, (2014) (PubMed).

Devereaux, DallArmi, Alcazar-Roman, Ogasawara, Zhou, Wang, Yamamoto, De Camilli, Di Paolo: "Regulation of mammalian autophagy by class II and III PI 3-kinases through PI3P synthesis." in: PLoS ONE, Vol. 8, Issue 10, pp. e76405, (2013) (PubMed).

Target Details for PIK3R4

Target: PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))

Alternative Name: PIK3R4

Background: Full Gene Name: phosphoinositide-3-kinase, regulatory subunit 4
Synonyms: MGC102700,VPS15,p150

Gene ID: 30849

NCBI Accession: NM_014602