RHOT1 antibody (AA 483-580)

Catalog No. ABIN565853

The Mouse Monoclonal anti-RHOT1 antibody has been validated for WB and ELISA. It is suitable to detect RHOT1 in samples from Human. There are 4+ publications available.

Quick Overview for RHOT1 antibody (AA 483-580) (ABIN565853)

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RHOT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 4H4

Product Details anti-RHOT1 Antibody

Binding Specificity: AA 483-580

Purpose: Mouse monoclonal antibody raised against a partial recombinant RHOT1.

Sequence: TEAEIICDVV CLVYDVSNPK SFEYCARIFK QHFMDSRIPC LIVAAKSDLH EVKQEYSISP TDFCRKHKMP PPQAFTCNTA DAPSKDIFVK LTTMAMYP

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: RHOT1 (NP_060777, 483 a.a. ~ 580 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-RHOT1 antibody (ABIN565853)

Su, Ji, Sun, Liu, Chen: "Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission." in: The Journal of biological chemistry, Vol. 289, Issue 3, pp. 1213-26, (2014) (PubMed).

Chen, Serrano, Mayhue, Wieden, Stankova, Boulay, Ilangumaran, Ramanathan: "GTPase of the immune-associated nucleotide-binding protein 5 (GIMAP5) regulates calcium influx in T-lymphocytes by promoting mitochondrial calcium accumulation." in: The Biochemical journal, Vol. 449, Issue 2, pp. 353-64, (2012) (PubMed).

Brickley, Pozo, Stephenson: "N-acetylglucosamine transferase is an integral component of a kinesin-directed mitochondrial trafficking complex." in: Biochimica et biophysica acta, Vol. 1813, Issue 1, pp. 269-81, (2011) (PubMed).

MacAskill, Brickley, Stephenson, Kittler: "GTPase dependent recruitment of Grif-1 by Miro1 regulates mitochondrial trafficking in hippocampal neurons." in: Molecular and cellular neurosciences, Vol. 40, Issue 3, pp. 301-12, (2009) (PubMed).

Target Details for RHOT1

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Alternative Name: RHOT1

Background: Full Gene Name: ras homolog gene family, member T1
Synonyms: ARHT1,FLJ11040,FLJ12633,MIRO-1

Gene ID: 55288

NCBI Accession: NM_018307