PMEPA1 antibody (AA 181-280)

Catalog No. ABIN566055

Product Details anti-PMEPA1 Antibody

Target: PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1 (PMEPA1))

Binding Specificity: AA 181-280

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PMEPA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Mouse monoclonal antibody raised against a partial recombinant TMEPAI.

Sequence: NRTIFDSDLM DSARLGGPCP PSSNSGISAT CYGSGGRMEG PPPTYSEVIG HYPGSSFQHQ QSSGPPSLLE GTRLHHTHIA PLESAAIWSK EKDKQKGHPL

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: TMEPAI (AAH15918, 181 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone: 2A12

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-PMEPA1 antibody (ABIN566055)

Ó hAinmhire, Quartuccio, Cheng, Ahmed, King, Burdette: "Mutation or loss of p53 differentially modifies TGF? action in ovarian cancer." in: PLoS ONE, Vol. 9, Issue 2, pp. e89553, (2014) (PubMed).

Singha, Pandeswara, Geng, Lan, Venkatachalam, Saikumar: "TGF-? induced TMEPAI/PMEPA1 inhibits canonical Smad signaling through R-Smad sequestration and promotes non-canonical PI3K/Akt signaling by reducing PTEN in triple negative breast cancer." in: Genes & cancer, Vol. 5, Issue 9-10, pp. 320-36, (2014) (PubMed).

Costea, Hills, Osman, Thurlow, Kalna, Huang, Pena Murillo, Parajuli, Suliman, Kulasekara, Johannessen, Partridge: "Identification of two distinct carcinoma-associated fibroblast subtypes with differential tumor-promoting abilities in oral squamous cell carcinoma." in: Cancer research, Vol. 73, Issue 13, pp. 3888-901, (2013) (PubMed).

Hu, He, Wang, Yuan, Liu, Ji, Song: "TMEPAI regulates EMT in lung cancer cells by modulating the ROS and IRS-1 signaling pathways." in: Carcinogenesis, Vol. 34, Issue 8, pp. 1764-72, (2013) (PubMed).

Hirokawa, Takagi, Uchida, Kozuka, Yoneda, Watanabe, Shiraishi: "High level expression of STAG1/PMEPA1 in an androgen-independent prostate cancer PC3 subclone." in: Cellular & molecular biology letters, Vol. 12, Issue 3, pp. 370-7, (2012) (PubMed).

Watanabe, Itoh, Goto, Ohnishi, Inamitsu, Itoh, Satoh, Wiercinska, Yang, Shi, Tanaka, Nakano, Mommaas, Shibuya, Ten Dijke, Kato: "TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling." in: Molecular cell, Vol. 37, Issue 1, pp. 123-34, (2010) (PubMed).

Singha, Yeh, Venkatachalam, Saikumar: "Transforming growth factor-beta (TGF-beta)-inducible gene TMEPAI converts TGF-beta from a tumor suppressor to a tumor promoter in breast cancer." in: Cancer research, Vol. 70, Issue 15, pp. 6377-83, (2010) (PubMed).

Li, Xu, Masuda, Raymundo, McLeod, Dobi, Srivastava: "A feedback loop between the androgen receptor and a NEDD4-binding protein, PMEPA1, in prostate cancer cells." in: The Journal of biological chemistry, Vol. 283, Issue 43, pp. 28988-95, (2008) (PubMed).

Target Details for PMEPA1

Target: PMEPA1 (Prostate Transmembrane Protein, Androgen Induced 1 (PMEPA1))

Alternative Name: PMEPA1 (PMEPA1 Products)

Background: Full Gene Name: prostate transmembrane protein, androgen induced 1
Synonyms: STAG1,TMEPAI

Gene ID: 56937

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway