SMURF1 antibody (AA 165-268)

Catalog No. ABIN566098

This Mouse Monoclonal antibody specifically detects SMURF1 in WB, ELISA, IF, IHC (p) and IP. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for SMURF1 antibody (AA 165-268) (ABIN566098)

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMURF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: 1D7

Product Details anti-SMURF1 Antibody

Binding Specificity: AA 165-268

Purpose: Mouse monoclonal antibody raised against a partial recombinant SMURF1.

Sequence: DSGPGRPLSC FMEEPAPYTD STGAAAGGGN CRFVESPSQD QRLQAQRLRN PDVRGSLQTP QNRPHGHQSP ELPEGYEQRT TVQGQVYFLH TQTGVSTWHD PRIP

Cross-Reactivity: Human, Mouse

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: SMURF1 (NP_065162, 165 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-SMURF1 antibody (ABIN566098)

Wang, Zhang, Weng, Qiao, Ma, Xiao, Yu, Pan, Sun et al.: "Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in ..." in: The Journal of biological chemistry, Vol. 288, Issue 47, pp. 33667-81, (2013) (PubMed).

Murakami, Mathew, Huang, Farahani, Peng, Olson, Etlinger: "Smurf1 ubiquitin ligase causes downregulation of BMP receptors and is induced in monocrotaline and hypoxia models of pulmonary arterial hypertension." in: Experimental biology and medicine (Maywood, N.J.), Vol. 235, Issue 7, pp. 805-13, (2010) (PubMed).

Target Details for SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Alternative Name: SMURF1

Background: Full Gene Name: SMAD specific E3 ubiquitin protein ligase 1
Synonyms: KIAA1625

Gene ID: 57154

NCBI Accession: NM_020429

Pathways: Regulation of Cell Size