Hemoglobin Subunit beta antibody (AA 1-145)

Catalog No. ABIN5662058

This anti-Hemoglobin Subunit beta antibody is a Mouse Monoclonal antibody detecting Hemoglobin Subunit beta in WB, IHC, IP and ICC. Suitable for Sheep.

Quick Overview for Hemoglobin Subunit beta antibody (AA 1-145) (ABIN5662058)

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Reactivity: Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hemoglobin Subunit beta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: D4

Product Details anti-Hemoglobin Subunit beta Antibody

Binding Specificity: AA 1-145

Purpose: Monoclonal Antibody to Hemoglobin Beta (HBb)

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFELRRQAC- MLTAEEKAAV TGFWGKVKVD EVGAEALGRL LVVYPWTQRF FEHFGDLSNA DAVMNNPKVK AHGKKVLDSF SNGMKHLDDL KGTFAQLSEL HCDKLHVDPE NFRLLGNVLV VVLARHHGNE FTPVLQADFQ KVVAGVANAL AHKYH

Specificity: The antibody is a mouse monoclonal antibody raised against HBb. It has been selected for its ability to recognize HBb in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Native Hemoglobin Beta (HBb)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-5 μg/mLImmunohistochemistry: 5-30 μg/mLImmunocytochemistry: 5-30 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Hemoglobin Subunit beta

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Alternative Name: Hemoglobin Beta

Background: HB-B, HBD, CD113t-C, Beta Globin, Spinorphin