DNAI2 antibody (AA 1-593)

Catalog No. ABIN566265

This anti-DNAI2 antibody is a Mouse Monoclonal antibody detecting DNAI2 in WB, ELISA and IF. Suitable for Human. This Primary Antibody has been cited in 6+ publications.

Quick Overview for DNAI2 antibody (AA 1-593) (ABIN566265)

Target: DNAI2 (Dynein, Axonemal, Intermediate Chain 2 (DNAI2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNAI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 1C8

Product Details anti-DNAI2 Antibody

Binding Specificity: AA 1-593

Purpose: Mouse monoclonal antibody raised against a full length recombinant DNAI2.

Sequence: MEIVYVYVKK RSEFGKQCNF SDRQAELNID IMPNPELAEQ FVERNPVDTG IQCSISMSEH EANSERFEME TRGVNHVEGG WPKDVNPLEL EQTIRFRKKV EKDENYVNAI MQLGSIMEHC IKQNNAIDIY EEYFNDEEAM EVMEEDPSAK TINVFRDPQE IKRAATHLSW HPDGNRKLAV AYSCLDFQRA PVGMSSDSYI WDLENPNKPE LALKPSSPLV TLEFNPKDSH VLLGGCYNGQ IACWDTRKGS LVAELSTIES SHRDPVYGTI WLQSKTGTEC FSASTDGQVM WWDIRKMSEP TEVVILDITK KEQLENALGA ISLEFESTLP TKFMVGTEQG IVISCNRKAK TSAEKIVCTF PGHHGPIYAL QRNPFYPKNF LTVGDWTARI WSEDSRESSI MWTKYHMAYL TDAAWSPVRP TVFFTTRMDG TLDIWDFMFE QCDPTLSLKD NGCLIACGSQ LGTTTLLEVS PGLSTLQRNE KNVASSMFER ETRREKILEA RHREMRLKEK GKAEGRDEEQ TDEELAVDLE ALVSKAEEEF FDIIFTELKK KEADAIKLTP VPQQPSPEED QVVEEGEEAA GEEGDEEVEE DLA

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: DNAI2 (AAH39582, 1 a.a. ~ 593 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-DNAI2 antibody (ABIN566265)

Ben Khelifa, Coutton, Zouari, Karaouzène, Rendu, Bidart, Yassine, Pierre, Delaroche, Hennebicq, Grunwald, Escalier, Pernet-Gallay, Jouk, Thierry-Mieg, Touré, Arnoult, Ray: "Mutations in DNAH1, which encodes an inner arm heavy chain dynein, lead to male infertility from multiple morphological abnormalities of the sperm flagella." in: American journal of human genetics, Vol. 94, Issue 1, pp. 95-104, (2014) (PubMed).

Dong, Shinohara, Botilde, Nabeshima, Asai, Fukumoto, Hasegawa, Matsuo, Takeda, Shiratori, Nakamura, Hamada: "Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm." in: The Journal of cell biology, Vol. 204, Issue 2, pp. 203-13, (2014) (PubMed).

Diggle, Moore, Mali, zur Lage, Ait-Lounis, Schmidts, Shoemark, Garcia Munoz, Halachev, Gautier, Yeyati, Bonthron, Carr, Hayward, Markham, Hope, von Kriegsheim, Mitchison, Jackson, Durand, Reith et al.: "HEATR2 plays a conserved role in assembly of the ciliary motile apparatus. ..." in: PLoS genetics, Vol. 10, Issue 9, pp. e1004577, (2014) (PubMed).

Tarkar, Loges, Slagle, Francis, Dougherty, Tamayo, Shook, Cantino, Schwartz, Jahnke, Olbrich, Werner, Raidt, Pennekamp, Abouhamed, Hjeij, Köhler, Griese, Li, Lemke, Klena, Liu, Gabriel, Tobita et al.: "DYX1C1 is required for axonemal dynein assembly and ciliary motility. ..." in: Nature genetics, Vol. 45, Issue 9, pp. 995-1003, (2013) (PubMed).

Loges, Olbrich, Fenske, Mussaffi, Horvath, Fliegauf, Kuhl, Baktai, Peterffy, Chodhari, Chung, Rutman, OCallaghan, Blau, Tiszlavicz, Voelkel, Witt, Zietkiewicz, Neesen, Reinhardt, Mitchison, Omran: "DNAI2 mutations cause primary ciliary dyskinesia with defects in the outer dynein arm." in: American journal of human genetics, Vol. 83, Issue 5, pp. 547-58, (2008) (PubMed).

Omran, Kobayashi, Olbrich, Tsukahara, Loges, Hagiwara, Zhang, Leblond, OToole, Hara, Mizuno, Kawano, Fliegauf, Yagi, Koshida, Miyawaki, Zentgraf, Seithe, Reinhardt, Watanabe, Kamiya, Mitchell, Takeda: "Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins." in: Nature, Vol. 456, Issue 7222, pp. 611-6, (2008) (PubMed).

Target Details for DNAI2

Target: DNAI2 (Dynein, Axonemal, Intermediate Chain 2 (DNAI2))

Alternative Name: DNAI2

Background: Full Gene Name: dynein, axonemal, intermediate chain 2
Synonyms: CILD9

Gene ID: 64446