Nanog antibody (AA 98-195)

Catalog No. ABIN566403

This anti-Nanog antibody is a Mouse Monoclonal antibody detecting Nanog in WB and ELISA. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Nanog antibody (AA 98-195) (ABIN566403)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2C11

Product Details anti-Nanog Antibody

Binding Specificity: AA 98-195

Purpose: Mouse monoclonal antibody raised against a partial recombinant NANOG.

Sequence: TRTVFSSTQL CVLNDRFQRQ KYLSLQQMQE LSNILNLSYK QVKTWFQNQR MKSKRWQKNN WPKNSNGVTQ KASAPTYPSL YSSYHQGCLV NPTGNLPM

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: NANOG (NP_079141, 98 a.a. ~ 195 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Nanog antibody (ABIN566403)

Liu, Badeaux, Choy, Chandra, Shen, Jeter, Rycaj, Lee, Person, Liu, Chen, Shen, Jung, Qin, Tang: "Nanog1 in NTERA-2 and recombinant NanogP8 from somatic cancer cells adopt multiple protein conformations and migrate at multiple M.W species." in: PLoS ONE, Vol. 9, Issue 3, pp. e90615, (2014) (PubMed).

Eberle, Pless, Braun, Dingermann, Marschalek: "Transcriptional properties of human NANOG1 and NANOG2 in acute leukemic cells." in: Nucleic acids research, Vol. 38, Issue 16, pp. 5384-95, (2010) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG

Background: Full Gene Name: Nanog homeobox
Synonyms:

Gene ID: 79923

NCBI Accession: NM_024865

Pathways: Stem Cell Maintenance