PD-L1 antibody (PE)

Catalog No. ABIN5665753

This anti-PD-L1 antibody is a Mouse Monoclonal antibody detecting PD-L1 in FACS. Suitable for Human and Non-Human Primate.

Quick Overview for PD-L1 antibody (PE) (ABIN5665753)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 29E-2A3

Product Details anti-PD-L1 Antibody (PE)

Purpose: Anti-Hu CD274 PE

Specificity: The mouse monoclonal antibody 29E.2A3 recognizes an extracellular epitope of CD274 / PD-L1 (also known as B7-H1), a 40 kDa type I transmembrane protein expressed by dendritic cells, activated T cells, activated monocytes, and in various tissues, above all in heart and skeletal muscle, placenta and lung, and in many cancer cells, including T cell lymphomas, melanomas, and glioblastomas.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Full length human CD274

Isotype: IgG2b kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274

Background: CD274 Molecule,CD274 / PD-L1 (programmed death ligand-1), also known as B7-H1, is a member of the B7 family of regulatory proteins. It can act as both costimulatory and coinhibitory molecule for T cells. Interaction with its receptor CD279 (PD1) appears to be important in the maintenance of peripheral tolerance and in prevention of tumor rejection. Even pathogens (e.g. Schistosoma) may exploit CD274 to evade an immune response. Besides CD279, existence of other receptor(s) for CD274 is likely.,B7H1, PDL1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7-H1

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints