Recombinant V alpha TCR antibody

Catalog No. ABIN5668025

The Mouse Monoclonal anti-V alpha TCR antibody (Clone 34) (ABIN5668025) specifically detects V alpha TCR in ELISA, FACS and WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant V alpha TCR antibody (ABIN5668025)

Target: V alpha TCR

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB)

Clone: 34

Product Details

Purpose: Anti-Vα TCR [34], Mouse IgG1, kappa

Specificity: This antibody binds to the T-cell receptor alpha chains.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: scFv

Purification: Protein A affinity purified

Immunogen: Purified recombinant soluble TCR from the T-cell hybridoma 85.33, corresponding to V alpha 11

Isotype: IgG1 kappa

Application Details

Application Notes: The antibody binds specifically to the recombinant and native variable domain of the α chain which comprises the T-cell receptor (TCR). The TCR is a disulfide-linked heterodimeric protein expressed on the surface of T-cells in complex with CD3 and ζ-chain molecules. The receptor functions in the recognition of MHC molecule and the subsequent activation of T-cells. The monoclonal antibody 34 recognises Vα regions of TCRs expressed in the 85.33 arthritogenic T-cell hybridoma and can be used to analyse TCR V gene expression and for immunotherapy in T cell mediated autoimmunity.

Comment:

This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: V alpha TCR

Background: TCR variable alpha chain, T-cell receptor variable alpha chain

UniProt: P01848