Recombinant TNF alpha (Adalimumab Biosimilar) antibody

Catalog No. ABIN5668145

This Human Monoclonal antibody specifically detects TNF alpha (Adalimumab Biosimilar) in ELISA, BR, IF and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Recombinant TNF alpha (Adalimumab Biosimilar) antibody (ABIN5668145)

Target: TNF alpha (Adalimumab Biosimilar)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Human

Clonality: Monoclonal

Conjugate: This TNF alpha (Adalimumab Biosimilar) antibody is un-conjugated

Application: ELISA, Blocking Reagent (BR), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: D2E7

Product Details anti-TNF alpha (Adalimumab Biosimilar) Antibody

Purpose: Anti-TNF alpha [D2E7 (Adalimumab)], Human IgG1, kappa

Specificity: Binds to soluble TNF-alpha, but not to lymphotoxin (TNF beta).

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Purity: > 98 % as determined by SDS-PAGE

Endotoxin Level: Endotoxin is < 1.0 EU/mg as determined by the LAL method

Immunogen: This antibody was generated by affinity maturation from the 2SD4 scFv, which in turn was selected on human TNF alpha by guided phage-display technology, using the murine anti-hTNF alpha antibody Mab32 as a template (c.f. also US Patent 6090382).

Isotype: IgG1 kappa

Application Details

Application Notes: Adalimumab binds specifically to TNF and neutralizes the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors. Adalimumab, unlike Infliximab (Ab00146), was shown to be cross-reactive with mouse TNF alpha (Arnoult 2017, PMID: 28584008).

Comment:

NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

References for anti-TNF alpha (Adalimumab Biosimilar) antibody (ABIN5668145)

Gupta, Flaskamp, Roentgen, Juergens, Armero-Gimenez, Albrecht, Hemmerich, Arfi, Neuser, Spiegel, Schillberg, Yeliseev, Song, Qiu, Williams, Finnern: "Scaling eukaryotic cell-free protein synthesis achieved with the versatile and high-yielding tobacco BY-2 cell lysate." in: Biotechnology and bioengineering, (2023) (PubMed).

Target Details for TNF alpha (Adalimumab Biosimilar)

Target: TNF alpha (Adalimumab Biosimilar)

Target Type: Biosimilar

Background: Tumor necrosis factor alpha

UniProt: P01375