Recombinant CD3 epsilon antibody

Catalog No. ABIN5668185

The Rabbit Chimeric anti-CD3 epsilon antibody has been validated for FACS and Act. It is suitable to detect CD3 epsilon in samples from Human.

Quick Overview for Recombinant CD3 epsilon antibody (ABIN5668185)

Target: CD3 epsilon (CD3E)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Activation (Act)

Clone: 12F6

Product Details anti-CD3 epsilon Antibody

Purpose: Anti-CD3 epsilon [12F6], Rabbit IgG, kappa

Specificity: This antibody binds to human CD3.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: Human CD3

Isotype: IgG kappa

Application Details

Application Notes: This antibody competes for CD3 binding with the therapeutic antibody OKT3 (Ab00122). 12F6 binding to CD3 on human T-cells stimulates the secretion of TNF-alpha, INF-gamma and IL-10. Humanized versions of this antibody are also available.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3 epsilon

Background: Leu4/T3, ET-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway