Recombinant ErbB2/Her2 (Trastuzumab Biosimilar) antibody

Catalog No. ABIN5668226

This Human Monoclonal antibody specifically detects ErbB2/Her2 (Trastuzumab Biosimilar) in FACS, ELISA and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Recombinant ErbB2/Her2 (Trastuzumab Biosimilar) antibody (ABIN5668226)

Target: ErbB2/Her2 (Trastuzumab Biosimilar)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Human

Clonality: Monoclonal

Conjugate: This ErbB2/Her2 (Trastuzumab Biosimilar) antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC)

Clone: 4D5-8

Product Details anti-ErbB2/Her2 (Trastuzumab Biosimilar) Antibody

Purpose: Anti-erbB-2 (Her-2/neu) [4D5-8 (trastuzumab)], Human IgG1, kappa

Specificity: Oncogenic protein Her-2/neu (erbB-2), a member of the family of epidermal growth factor (EGF) receptors, overexpressed in 30 % of invasive breast cancers and 70 % of ductal carcinomata in situ, in a small percentage of melanomas, and in other malignancies originating from various organs, including ovary, kidney, colon, and bladder.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Purity: > 98 % as determined by SDS-PAGE

Endotoxin Level: Endotoxin is < 1.0 EU/mg as determined by the LAL method

Immunogen: A431 cells (human epidermoid carcinoma) (over)expressing EGFR.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody binds to erbB-2/Her-2/neu has therapeutic indications particularly in the treatment of breast cancer (c.f. Herceptin). It can also be used to detect HER-2 by flow cytometry or IHC (Glazyrin 2007).

Comment:

NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

References for anti-ErbB2/Her2 (Trastuzumab Biosimilar) antibody (ABIN5668226)

Maisel, Broka, Atwell, Bunch, Kupp, Singh, Mehta, Schroeder: "Stapled EGFR peptide reduces inflammatory breast cancer and inhibits additional HER-driven models of cancer." in: Journal of translational medicine, Vol. 17, Issue 1, pp. 201, (2020) (PubMed).

Target Details for ErbB2/Her2 (Trastuzumab Biosimilar)

Target: ErbB2/Her2 (Trastuzumab Biosimilar)

Target Type: Biosimilar

Background: CD340, Metastatic lymph node gene 19 protein (MLN 19), Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, p185HER2, EGFR

UniProt: P04626