Recombinant ITGA5 (Volociximab Biosimilar) antibody

Catalog No. ABIN5668271

This Rabbit Chimeric antibody specifically detects in BR, ELISA, FACS, IF and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant ITGA5 (Volociximab Biosimilar) antibody (ABIN5668271)

Target: ITGA5 (Volociximab Biosimilar)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Blocking Reagent (BR), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: M200

Product Details

Purpose: Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)], Rabbit IgG, kappa

Specificity: The antibody binds to human α5β1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG4

Purification: Protein A affinity purified

Purity: > 98 % as determined by SDS-PAGE

Endotoxin Level: Endotoxin is < 1.0 EU/mg as determined by the LAL method

Immunogen: Heterodimeric alpha5beta1Fc fusion protein

Isotype: IgG kappa

Application Details

Application Notes: The antibody binds specifically to α5β1 integrin, which is part of the superfamily of glycoprotein transmembrane receptors for ligands such as fibronectin, vitronectin, laminins and collagens. The α5β1 integrin receptor plays a key role in cellular processes such as inflammation, cell proliferation, angiogenesis and tumour metastasis. Comprising the variable domain of the original mouse antibody, a chimeric mouse:human IgG4 version of this antibody has been tested in vivo: In cynomolgus monkeys with laser-induced choroidal neovascularization (CNV), in rabbit models and in human volunteers. The antibody reached Phase II clinical trials with PDL Biopharma and Biogen-Idec.

Comment:

NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: ITGA5 (Volociximab Biosimilar)

Target Type: Biosimilar

Background: Fibronectin receptor, CD49 antigen-like family member E, Integrin alpha-F, VLA-5, CD49e.

UniProt: P08648