SAE1 antibody (AA 1-100) (HRP)

Catalog No. ABIN5674969

The Rabbit Polyclonal anti-SAE1 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect SAE1 in samples from Mouse.

Quick Overview for SAE1 antibody (AA 1-100) (HRP) (ABIN5674969)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAE1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SAE1 Antibody (HRP)

Binding Specificity: AA 1-100

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SAE1,Isoform 2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: SAE1

Background:

Synonyms: SUMO-activating enzyme subunit 1, SAE1, Ubiquitin-like 1-activating enzyme E1A, SAE1, AOS1, SUA1, UBLE1A

Background: The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Gene ID: 10055

UniProt: Q9UBE0