DAPK3 antibody (pThr265)

Catalog No. ABIN5675187

This Rabbit Polyclonal antibody specifically detects DAPK3 in WB, ELISA, IF (cc), IF (p), ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for DAPK3 antibody (pThr265) (ABIN5675187)

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAPK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DAPK3 Antibody

Binding Specificity: pThr265

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human ZIP Kinase around the phosphorylation site of Thr265

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DAPK3

Target: DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))

Alternative Name: ZIP Kinase

Background:

Synonyms: Death-associated protein kinase 3, DAPK3, DAP kinase 3, Dlk, MYPT1 kinase, Zipper-interacting protein kinase, ZIP-kinase, ZIPK

Background: Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.

Gene ID: 1613

UniProt: O43293